Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice

Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice

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Abstract Previously, genetic lineage tracing based on the mesothelial marker Wt1, appeared to show that peritoneal mesothelial cells have a range of differentiative capacities and are the direct progenitors of vascular smooth muscle in the intestine.However, it was not clear whether this was a here temporally limited process or continued throughout postnatal life.Here, using a conditional Wt1-based genetic lineage tracing approach, we demonstrate that the postnatal and adult peritoneum covering intestine, mesentery and body wall only maintained itself and failed to contribute to other visceral tissues.Pulse-chase experiments of up to 6 months revealed that Wt1-expressing cells remained confined to the peritoneum and failed to differentiate into cellular components of blood vessels or other tissues underlying the peritoneum.

Our data confirmed that the Wt1-lineage system also labelled submesothelial cells.Ablation of Wt1 in adult mice did not result in changes to the intestinal wall architecture.In the heart, we observed that Wt1-expressing cells maintained powell and mahoney bloody mary mix the epicardium and contributed to coronary vessels in newborn and adult mice.Our results demonstrate that Wt1-expressing cells in the peritoneum have limited differentiation capacities, and that contribution of Wt1-expressing cells to cardiac vasculature is based on organ-specific mechanisms.